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• The cDNA libraries were subjected to the NovaSeq 6,000 system (Illumina, CA, United States), according to commercially available protocols.
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• Precipitates were eluted from the magnetic bead-antibody-antigen complex using the elution buffer containing NuPAGE LDS sample buffer (ThermoFisher) and NuPAGE sample reducing agent (ThermoFisher) for 10 min at 70 °C.
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• From the result of the overlap genes between TCGA-4 Cs and RNA-seq, four genes were found to be influenced by PRR11 downregulation, including PTTG1, BRCA2, ATAD2, and BRIP1.

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Apart from that, you will get your own manager, who can help you with emerging problems. Become a member of the affiliate program, create your own referral link, and invite more potential users to join the Rich11 community. Copyright © 2022 Zhang, He, Qiu, Wei, Gong, Xian, Chen, Cui, Fu, Zhang, Hu, Zhang, Lin and Du.

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PRR11 knockdown re-sensitized fulvestrant-resistant (FulvR) MCF7 cells to fulvestrant (Fig. 3i and Supplementary Fig. 3c). A similar result was observed in tamoxifen-resistant (TamR) MCF7 and TamR HCC1428 cells (Supplementary Fig. 3d). These data suggest PRR11 promotes resistance to antiestrogens and its downregulation enhances the action of ER-targeted therapies against ER+ breast cancer cells. Unexpectedly, we did not find significant changes in the expression of cyclin genes with downregulation of PRR11, except cyclin D3 (CCND3) in BxPC3. Moreover, the early growth response protein 1 (EGR1) gene was also significantly suppressed after interfering PRR11 in NCI-H460, which is consistent with the result of a previous study (Chen et al., 2015). From the result of the overlap genes between TCGA-4 Cs and RNA-seq, four genes were found to be influenced by PRR11 downregulation, including PTTG1, BRCA2, ATAD2, and BRIP1.

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In addition, the rescue assay verified PRR11/PTTG1 promoting cell proliferation in NCI-H460 (Supplementary Figure S4B). Thus, PRR11 silencing in pan-cancers could suppress the expression level of PTTG1. PRR11 has been found highly expressed in various cancer types and strongly correlated with their poor outcome. In the present study, PRR11 was highly expressed in 19 out of 33 kinds of cancers in TCGA.

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